CD19-Luc K562
CBP74668
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| I. Background | |
| CD19（Cluster of Differentiation 19）是一种跨膜糖蛋白，属于免疫球蛋白超家族成员， 主要表达于 B 淋巴细胞谱系细胞表面，包括前 B 细胞、成熟 B 细胞及大多数 B 细胞恶性肿 瘤细胞。其功能是作为 B 细胞受体（BCR）复合物的共受体，参与 B 细胞活化、增殖和分 化信号的传导。CD19 作为 B 细胞恶性肿瘤的核心靶点，有助于了 CAR-T 等精准免疫治疗的 开展，显著改善了患者预后。 | |
| II. Description | |
| CD19-Luc K562 细胞，在K562细胞的基础上，稳定表达 CD19。 | |
| III. Introduction | |
| Expressed gene: | CD19-Luc |
| Host Cell: | K562 |
| Stability: | 32 passages (in-house test, that not means the cell line will be instable beyond the passages we tested.) |
| Freeze Medium: | 90% FBS+10% DMSO |
| Culture Medium: | RPMI-1640 +10%FBS +5μg/ml Blasticidin +1 μg/ml Puromycin |
| Mycoplasma Testing: | Negative |
| Storage: | Liquid nitrogen |
| Application(s): | Binding Assay,FACS |
| IV. Representative Data | |
 Figure 1. Recombinant CD19-Luc K562 constitutively expressing CD19.  Figure 2. Detect Luciferase assay by Ultra Luciferase Detection Kit CBPH0001（we strongly recommend purchasing from Cobioer).The luminescence signal can be robustly and stably detected in CD19-Luc K562 cells, and CD19-Luc K562 cells maintain stable luminescence intensity for at least 32 passages. |
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